BIOASSAY OF INSULIN BIOASSAY OF D-TUBOCURARINE MERITS OF BIOASSAY DEMERITS OF BIOASSAY REFRENCES. is being done through. Bioassay, official method, adrenaline, digitalis, d-tubocurarine, heparin, insulin, oxytocin, gonadotropin, chorionic gonadotropin, urokinase, streptokinase. Second 4 x 4 latin square bioassay was performed using a series: with a The presence of d-tubocurarine was demonstrated in the Peruvian.

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Rabbit head drop method for the bioassay of d-tubocurarine. Bioassay of D- Tubocurarine.

Minimum 8 rabbits are used. You do not have the permission to view this presentation. A frog is pithed and laid on its back on a cork covered board to which it is pinned. Share to Twitter Share to Facebook. Receptors The rectus abdominis muscle of frog mainly rich of Nicotinic receptor. God Bless you man. It is also useful for bioassay of Acetylcholine.

Suitable dose of d-tubocurarine bioassau 0. Functions It is an important postural muscle. Bioassya changes to Flash or non-Flash embed. In order to view it, please contact the author of the presentation.


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A linear response will be obtained. Animals should be free from disease, obtained from a healthy colony and should be accustomed with the experimental procedure.

Newer Post Older Post Home. Location The rectus abdominis is a long flat muscle, which extends along the whole length of the front of the abdomen.

Head drop after injection. Acetylcholine contraction is recorded on slow moving drum for 90 sec.

The muscle is stabilized for min. Bioassay of D-Tubocurarine 1. It is a perfect in vitro model. Location of Rectus muscle. First group will receive standard sample and the second group will receive the sample under test. Those rabbits which received the standard sample on the first day will bioassaay given test sample on the second day of expt.

d-tubocurzrine The head should be freely movable. Rabbits recover immediately from the effect of curarization. The total amount of test sample required to produce the endpoint is compared with the total amount of the standard sample required to produce similar endpoint.


Cross-over test is carried out to minimise biological error due to animal variation. They are divided into two groups each containing 4 rabbits. The responses are recorded using isotonic frontal writing lever with 1 G.

College of Pharmacy, Ahmedabad.

PHARMACOLOGY: Bioassay of D-Tubocurarine

Ringer Solution 11 Components Amount gm Nacl 6. Saturday, 12 May Bioassay of D-Tubocurarine. Blood supply to Rectus muscle. Jyotirmoy Das Choudhury M.


Injection should be given at a rate of 0. I enjoy, result in I found exactly what I used to be looking for.

Pharm 1 st semester Phamacology Under the Guidance of Mr.